recombinant human ctcf protein Search Results


recombinant human ctcf protein  (Novus Biologicals)
90
Novus Biologicals recombinant human ctcf protein
(A) Supershift EMSA was performed using <t>CTCF</t> antibody. CTCF antibody (1 μg) was incubated with 0.5 μg <t>recombinant</t> human CTCF protein prior to the addition of the binding buffer and probe. G and A represent labelled probes containing the white eggshell and blue eggshell allele, respectively, and G cold and A cold represent cold probes containing the white eggshell and blue eggshell allele, respectively. (B) ChIP-qPCR analysis of CTCF binding on variation M5 in uteruses from blue-eggshelled and white-eggshelled ducks. Chromatin extracts from four individuals of each genotype were immunoprecipitated with CTCF antibody. ChIP-enriched DNA was quantified by qPCR with primers specific for variation M5. Rabbit IgG was used as a negative control. Values of immunoprecipitated samples were normalized to that of the input DNA. Data are presented as mean±SD from four independent individuals.
Recombinant Human Ctcf Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human ctcf protein/product/Novus Biologicals
Average 90 stars, based on 1 article reviews
recombinant human ctcf protein - by Bioz Stars, 2026-03
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recombinant human ctcf gst (n-term) protein  (Bio-Techne corporation)
90
Bio-Techne corporation recombinant human ctcf gst (n-term) protein
(A) Supershift EMSA was performed using <t>CTCF</t> antibody. CTCF antibody (1 μg) was incubated with 0.5 μg <t>recombinant</t> human CTCF protein prior to the addition of the binding buffer and probe. G and A represent labelled probes containing the white eggshell and blue eggshell allele, respectively, and G cold and A cold represent cold probes containing the white eggshell and blue eggshell allele, respectively. (B) ChIP-qPCR analysis of CTCF binding on variation M5 in uteruses from blue-eggshelled and white-eggshelled ducks. Chromatin extracts from four individuals of each genotype were immunoprecipitated with CTCF antibody. ChIP-enriched DNA was quantified by qPCR with primers specific for variation M5. Rabbit IgG was used as a negative control. Values of immunoprecipitated samples were normalized to that of the input DNA. Data are presented as mean±SD from four independent individuals.
Recombinant Human Ctcf Gst (N Term) Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human ctcf gst (n-term) protein/product/Bio-Techne corporation
Average 90 stars, based on 1 article reviews
recombinant human ctcf gst (n-term) protein - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ctcf protein  (OriGene)
90
OriGene ctcf protein
(A) Supershift EMSA was performed using <t>CTCF</t> antibody. CTCF antibody (1 μg) was incubated with 0.5 μg <t>recombinant</t> human CTCF protein prior to the addition of the binding buffer and probe. G and A represent labelled probes containing the white eggshell and blue eggshell allele, respectively, and G cold and A cold represent cold probes containing the white eggshell and blue eggshell allele, respectively. (B) ChIP-qPCR analysis of CTCF binding on variation M5 in uteruses from blue-eggshelled and white-eggshelled ducks. Chromatin extracts from four individuals of each genotype were immunoprecipitated with CTCF antibody. ChIP-enriched DNA was quantified by qPCR with primers specific for variation M5. Rabbit IgG was used as a negative control. Values of immunoprecipitated samples were normalized to that of the input DNA. Data are presented as mean±SD from four independent individuals.
Ctcf Protein, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ctcf protein/product/OriGene
Average 90 stars, based on 1 article reviews
ctcf protein - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
the recombinant human ctcf proteins  (Promega)
90
Promega the recombinant human ctcf proteins
(A) Supershift EMSA was performed using <t>CTCF</t> antibody. CTCF antibody (1 μg) was incubated with 0.5 μg <t>recombinant</t> human CTCF protein prior to the addition of the binding buffer and probe. G and A represent labelled probes containing the white eggshell and blue eggshell allele, respectively, and G cold and A cold represent cold probes containing the white eggshell and blue eggshell allele, respectively. (B) ChIP-qPCR analysis of CTCF binding on variation M5 in uteruses from blue-eggshelled and white-eggshelled ducks. Chromatin extracts from four individuals of each genotype were immunoprecipitated with CTCF antibody. ChIP-enriched DNA was quantified by qPCR with primers specific for variation M5. Rabbit IgG was used as a negative control. Values of immunoprecipitated samples were normalized to that of the input DNA. Data are presented as mean±SD from four independent individuals.
The Recombinant Human Ctcf Proteins, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/the recombinant human ctcf proteins/product/Promega
Average 90 stars, based on 1 article reviews
the recombinant human ctcf proteins - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


(A) Supershift EMSA was performed using CTCF antibody. CTCF antibody (1 μg) was incubated with 0.5 μg recombinant human CTCF protein prior to the addition of the binding buffer and probe. G and A represent labelled probes containing the white eggshell and blue eggshell allele, respectively, and G cold and A cold represent cold probes containing the white eggshell and blue eggshell allele, respectively. (B) ChIP-qPCR analysis of CTCF binding on variation M5 in uteruses from blue-eggshelled and white-eggshelled ducks. Chromatin extracts from four individuals of each genotype were immunoprecipitated with CTCF antibody. ChIP-enriched DNA was quantified by qPCR with primers specific for variation M5. Rabbit IgG was used as a negative control. Values of immunoprecipitated samples were normalized to that of the input DNA. Data are presented as mean±SD from four independent individuals.

Journal: PLoS Genetics

Article Title: Two cis -regulatory SNPs upstream of ABCG2 synergistically cause the blue eggshell phenotype in the duck

doi: 10.1371/journal.pgen.1009119

Figure Lengend Snippet: (A) Supershift EMSA was performed using CTCF antibody. CTCF antibody (1 μg) was incubated with 0.5 μg recombinant human CTCF protein prior to the addition of the binding buffer and probe. G and A represent labelled probes containing the white eggshell and blue eggshell allele, respectively, and G cold and A cold represent cold probes containing the white eggshell and blue eggshell allele, respectively. (B) ChIP-qPCR analysis of CTCF binding on variation M5 in uteruses from blue-eggshelled and white-eggshelled ducks. Chromatin extracts from four individuals of each genotype were immunoprecipitated with CTCF antibody. ChIP-enriched DNA was quantified by qPCR with primers specific for variation M5. Rabbit IgG was used as a negative control. Values of immunoprecipitated samples were normalized to that of the input DNA. Data are presented as mean±SD from four independent individuals.

Article Snippet: CTCF antibody (1μg) was incubated with 0.5 μg recombinant human CTCF protein (Novus biologicals, Littleton, USA) prior to addition of the binding buffer and probe.

Techniques: Incubation, Recombinant, Binding Assay, ChIP-qPCR, Immunoprecipitation, Negative Control

(A) Effect of knockdown of CTCF on the promoter activity of causative sites. The pGL3-Basic vectors containing the blue eggshell or white eggshell alleles inserted in their upstream region were transfected into DEF cells 6 hours after transfection of CTCF siRNA1 or negative control siRNA (scramble siRNA) or without siRNA. Cells were collected for luciferase activity analysis 30 hours after siRNA transfection. The group only transfected with the blue eggshell or white eggshell vector was used as control. Data represent the mean±SD from three biological repeats per vector. ** indicates P<0.01. (B) Schematic map of CpGs sites used in the DNA methylation analysis. The causative site M5 was referred as position +1. The black bars represented the CpG dinucleotides upstream or downstream of the predicted CTCF binding sites which were marked in grey background. (C) Heatmap of uterine DNA methylation levels of CpG sites in region A and B of blue-eggshelled and white-eggshelled ducks. The DNA methylation level was determined by sequencing 8 clones of bisulfite treated genomic DNA for each individual. The DNA methylation level of each CpG site was represented by the average methylation of 8 clones. The DNA methylation level from 0 to 1 is shown in the color from blue to red.

Journal: PLoS Genetics

Article Title: Two cis -regulatory SNPs upstream of ABCG2 synergistically cause the blue eggshell phenotype in the duck

doi: 10.1371/journal.pgen.1009119

Figure Lengend Snippet: (A) Effect of knockdown of CTCF on the promoter activity of causative sites. The pGL3-Basic vectors containing the blue eggshell or white eggshell alleles inserted in their upstream region were transfected into DEF cells 6 hours after transfection of CTCF siRNA1 or negative control siRNA (scramble siRNA) or without siRNA. Cells were collected for luciferase activity analysis 30 hours after siRNA transfection. The group only transfected with the blue eggshell or white eggshell vector was used as control. Data represent the mean±SD from three biological repeats per vector. ** indicates P<0.01. (B) Schematic map of CpGs sites used in the DNA methylation analysis. The causative site M5 was referred as position +1. The black bars represented the CpG dinucleotides upstream or downstream of the predicted CTCF binding sites which were marked in grey background. (C) Heatmap of uterine DNA methylation levels of CpG sites in region A and B of blue-eggshelled and white-eggshelled ducks. The DNA methylation level was determined by sequencing 8 clones of bisulfite treated genomic DNA for each individual. The DNA methylation level of each CpG site was represented by the average methylation of 8 clones. The DNA methylation level from 0 to 1 is shown in the color from blue to red.

Article Snippet: CTCF antibody (1μg) was incubated with 0.5 μg recombinant human CTCF protein (Novus biologicals, Littleton, USA) prior to addition of the binding buffer and probe.

Techniques: Knockdown, Activity Assay, Transfection, Negative Control, Luciferase, Plasmid Preparation, Control, DNA Methylation Assay, Binding Assay, Sequencing, Clone Assay, Methylation